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Fig. 2 | Journal of Nanobiotechnology

Fig. 2

From: Highly glycosylated MUC1 mediates high affinity L-selectin binding at the human endometrial surface

Fig. 2

Cell adhesion of HEC-1A and HEC-1B cells in the presence/absence of MUC1 specific siRNA. a A population wide screen (minimum 1000 cells) assessed for the expression of MUC1 at the protein level using IN Cell microscope, in the presence and absence of MUC1 siRNA for both cell lines. b and c Topography AFM images of both cell lines in the presence and absence of MUC1 specific siRNA, scale bar 20 µm (b – control and MUC1 siRNA image Z range is 4.5 µm; c – Control image Z range is 8.8 µm, MUC1 siRNA image Z range is 4.0 µm). (d  and e) HEC-1A and HEC-1B cell mechanical and adhesive properties, stiffness (kPa), adhesion (pN) and deformation (nm), achieved with AFM QNM imaging were assessed in the presence and absence of MUC1 siRNA. All data shown is based on a minimum of three biological repeats with three cellular areas per repeat (n = 9), analysed as parametric data using 2 tailed T test. Significance given as * p < 0.05, **p < 0.001

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