Fig. 3

Toxicity of LAMA-mic in different cell lines. a PrestoBlue™ assay in L-929 cells following incubation with respective polymers at indicated concentrations for 24 h. Dots represent values of single repetitions and lines represent logistic fit functions (n = 3). Stars indicate polymer concentrations used for N*/P 30 in transfection assays. b LDH-assay in HEK293T and K-562 cells following incubation with polyplexes of respective polymers and pDNA at N*/P 30 for 24 h. Values were calculated relative to the positive control Triton X-100 (100 % LDH release ≙ 0 % viability) and represent mean ± SD (n = 3). c Hemolysis as the amount of released hemoglobin calculated relative to 1 % Triton X-100 as positive control (100 % hemolysis). Human erythrocytes were washed and incubated with polymers at indicated concentrations in PBS of different pH values without serum. Values represent mean ± SD (n = 3) and are classified as slightly hemolytic between 2% and 5%, and as non- or hemolytic if lower or higher than 2% or 5%, respectively. Regarding significant differences, the main effects of the treatment were determined since there was no significant interaction of pH value and treatment