Fig. 6

Transfection efficiency and toxicity of LAMA-mic compared to control micelles. a Overview of the structures of the additional polymer set. b + c EGFP expression of viable cells was analyzed via flow cytometry following incubation of cells with polyplexes of mEGFP-N1 pDNA and polymers at N*/P 30, in respective growth medium (D10 or R10 with 10 mM HEPES) either for 24 h or for 24 h followed by splitting of cells and medium and further incubation for 48 h. Values represent mean ± SD (n = 3) of EGFP-positive cells relative (columns) and not relative (squares) to the respective LAMA-mic treatment. d + e Viability was determined according to the FSC/SSC scatter plot of flow cytometry. Values represent mean ± SD (n = 3). b + d HEK293T cells. c + e K-562 cells. Regarding significant differences, the main effects of the treatment were determined since there was no significant interaction of incubation time and treatment. *: significant difference to LAMA-mic II, N*/P 15 (p < 0.05). **: significant difference to LAMA-mic II, N*/P 15 (p < 0.01). ***: significant difference to LAMA-mic II, N*/P 15 (p < 0.001)