Skip to main content
Fig. 2 | Journal of Nanobiotechnology

Fig. 2

From: Regulating the immunosuppressive tumor microenvironment to enhance breast cancer immunotherapy using pH-responsive hybrid membrane-coated nanoparticles

Fig. 2

Electrophoretic gel assay, cellular uptake, and endosome escape of MC-PLGA@Met-CO2/FAM-siFGL1 NPs in 4T1 cells. a Agarose gel electrophoresis of siFGL1 and Met-CO2 binding affinity under different N/P ratios. b Electrophoretic gel assay of Met-CO2-siFGL1 (lane 1), PLGA@Met-CO2/siFGL1 (lane 2), and MC-PLGA@Met-CO2/siFGL1 (lane 3) (w/ w ratio = 1) at different pH values (pH = 7.4, 6.0 and 5.0). c RNase protection assay. Naked siRNA, siRNA encapsulated within the Met-CO2-siFGL1 + RNase A (or without adding RNase A) (lane 1), PLGA@Met-CO2/siFGL1 + RNase A (or without adding RNase A) (lane 2), MC-PLGA@Met-CO2/siFGL1 + RNase A (or without adding RNase A) (lane 3). d Flow cytometry analysis of the amount of FAM-siFGL1 internalized by 4T1 cells after 4 h of incubation. e Quantitative analysis of the relative fluorescence intensity of 4T1 cells (n = 3). f Confocal microscopy images of 4T1 cells incubated with FAM-siFGL1, PLGA@Met-CO2/FAM-siFGL1 NPs, or MC-PLGA@Met-CO2/FAM-siFGL1 NPs for 4 h. g Representative confocal microscopy images of 4T1 cells incubated with MC-PLGA@Met/FAM-siFGL1 NPs (without CO2), MC-PLGA@Met-CO2/siFGL1 NPs (with CO2) for 2 h and 4 h at 37 °C. The cell nuclei were stained using DAPI (blue), the endo/lysosomes were stained using LysoTracker Red (red), and siFGL1 was labeled with FAM (green). The scale bar is 20 μm. h Three-dimensional confocal images of 4T1 cells incubated with MC-PLGA@Met-CO2/FAM-siFGL1 NPs showing intracellular endosome escape behavior, with green representing siFGL1, red representing endosomes/lysosomes, and blue representing 4T1 cell nuclei

Back to article page