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Fig. 3 | Journal of Nanobiotechnology

Fig. 3

From: Regulating the immunosuppressive tumor microenvironment to enhance breast cancer immunotherapy using pH-responsive hybrid membrane-coated nanoparticles

Fig. 3

Release of Met from MC-PLGA@Met-CO2/FAM-siFGL1 NPs and cytotoxicity evaluation. a Release kinetics of Met from MC-PLGA@Met-CO2/FAM-siFGL1 NPs at different pH values (pH values of 7.4, 6.0, and 5.0). b After treatment with 10 mM Met mediated by various formulations for 48 h, cell cycles of 4T1 cells were examined using flow cytometry assay, and the graph depicts the quantification analysis for each group. c The biocompatibility of MC-PLGA NPs. d Apoptosis analysis of 4T1 cells after 48 h treatment with different formulations in the co-culture as evaluated by flow cytometry using Annexin V- FITC kit and PI staining. Corresponding statistics on the percentage of and necrotic cells are presented. e Western blot analysis of PD-L1, AMPK-α, Thr172 protein levels in 4T1 cells after 48-h treatment with different concentrations of Met mediated by MC-PLGA@Met-CO2/siFGL1 NPs. f Western blot analysis of FGL1 protein levels in 4T1 cells after 48-h treatment with different concentrations of siFGL1 mediated by MC-PLGA@Met-CO2/siFGL1 NPs

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