Fig. 3

MIF-Exo exhibited more significant protective effects on HUVECs and H9C2 cardiomycytes than MSC-Exo in vitro. Tube formation of HUVECs incubated with PBS, MIF-Exo, MSC-Exo and siMIF-Exo (a), and quantification analysis (b). Scale bar = 100 μm. (n = 3 biological replicates for each group). Migration was monitored for 12 h after scratching in HUVECs cultured with PBS, MIF-Exo, MSC-Exo and siMIF-Exo (c), and quantification analysis (d). Scale bar = 100 μm (n = 3 biological replicates for each group). In H/SD, DAPI nucleic acid stained for apoptosis of HUVECs after culturing with PBS, MIF-Exo, MSC-Exo and siMIF-Exo. Red point indicated apoptotic cells (e), and quantification analysis (f). Scale bar = 100 μm. (n = 3 biological replicates for each group; 5 random fields for each biological replicate). EdU positive cells were in PBS, MIF-Exo, MSC-Exo and siMIF-Exo (g), and quantification analysis (h). Scale bar = 100 μm. (n = 3 biological replicates for each group; 5 random fields for each biological replicate). In H/SD, apoptosis of H9C2 after incubating with PBS, MIF-Exo, MSC-Exo and siMIF-Exo (i), and quantification analysis (j). Continuous variables and categorical variables were described by means ± SEM and percentages. (n = 3 biological replicates for each group). *P < 0.05; **P < 0.01; ***P < 0.001