Fig. 4
Representative images of HCMVAD-169 plaques in treatments with G2-S16 dendrimer. a Optical and b confocal representative images of MRC-5 cells. Briefly, MRC-5 cells were plated and treated with G2-S16 dendrimer (10 µM) for 1 h and infected with 60 PFU for 2 h at 37 ºC. After 6 days incubation a MRC-5 cells were dyed with methylene blue and images were taken on a DMIL Leica microscope at 4X augment. b MRC-5 cells were treated with HCMVpp65 ALEXA FLUOR® 488 Antibody Conjugated for viral particles visualization, phalloidin ALEXA FLUOR® 555 Conjugated and DAPI for membrane and nucleus localization. NT: non-treated; CI: control of infection