Fig. 2

Impact of SiNP internalization on the viability and biological function of the HTR8 cells. a Images showing the biodistribution of FITC-SiNP internalized by the HTR8 cells (×10 magnification). The nuclei were stained with Hoechst in blue, SiNP was conjugated with FITC in green, and the cytoskeleton was stained with Tubulin in red. b Images showing the suppressed proliferation of the HTR8 cells, where the HTR8 cells were treated with SiNP without FITC conjugation, the nuclei were stained with DAPI in blue, the HTR8 cells in the proliferation stage were stained with EdU in green (×10 magnification). c CCK8 assay showing the attenuated cell viability of the SiNP-exposed HTR8 cells. d Quantitative analysis of the proliferation of the HTR8 cells. e Confocal laser scanning images showing the apoptotic HTR8 cells, where the apoptotic cells were labeled with white dots (×10 magnification). f Quantitative analysis of apoptotic HTR8 cells. g Images showing the wounds scratched in the HTR8 cells. The attenuated wound healing ability was represented by the reduced area of the wound (×10 magnification). h Quantitative analysis of wound healing ability of the HTR8 cells. i Optical microscopy images showing tubes formed by the HTR8 cells (×4 magnification). j Quantitative analysis of the total tube length formed by the HTR8 cells. The differences were compared using one-way ANOVA followed by SNK post-hoc test. *P < 0.05