Fig. 6

SiNP exposure elevated the expression of uterine cytokines in pregnant mice. a Immunohistochemical images showing the uterine expression of IL-1β, TGF-β, and TNF-α in mice administrated with 0.25 mg of SiNP (×20 magnification). The positive area of different cytokines was stained in brown. b Quantitative analysis showing the expression of IL-1β, TGF-β, and TNF-α at different observation time points. c Immunofluorescence images of uterine MCP-1 (×20 magnification). The nuclei were stained with DAPI in blue, and MCP-1 was stained with specific antibody in red. The differences were compared using one-way ANOVA followed by SNK post-hoc test. *P < 0.05