Fig. 3

a Evaluation the cytotoxicity of different concentrations of MPDA@SPIO in HepG2 and LO2, (b, c) HepG2 and LO2 transfected by MPDA@SPIO/SA-PEI/AFP-Fth (0; 2.5; 5; 10; 20/1.25/1 w/w/w) and PEI/AFP-Fth (1.25/1 w/w/). d Confocal Laser Scanning Microscope (CLSM) images of HepG2 and LO2 cells incubated with the MPDA@SPIO/SA-PEI/AFP-Fth (20/1.25/1 w/w/w) labeled ICG for 1, 2, 4 h. e The FAM labeled DNA of MPDA@SPIO/SA-PEI/FAM-DNA internalized by HepG2 at 1, 2 and 4 h. f Western blot assay for E-selectin in HepG2 and LO2. g Fluorescence images of HepG2 and LO2 cells stained with E-selectin antibody. Scale bar = 50 μm. (n = 3; ***p < 0.001)