Fig. 2

In vitro cell experiments. a Relative cell viabilities of LLC1 cells incubated in PMAA-Fe and Cyp-PMAA-Fe@MSCs with different concentrations for 24 h. b Confocal microscopic images of LLC1 cells incubated with Rhodamine-B-labeled PMAA-Fe@MSCs (red). Cell nucleus were dyed with DAPI (blue) and lysosomes were dyed with Lyso-tracker (green). c Flow cytometer data of LLC1 cells incubated in DMEM, Cyp-PMAA-Fe, Cyp-PMAA-Fe@RBCs and Cyp-PMAA-Fe@MSCs for 10, 30 and 60 min. d Confocal microscopic images of LLC1 cells treated with PBS, PMAA-Fe@MSCs and Cyp-PMAA-Fe@MSCs with/without 808 nm laser. Live and dead cells were dyed with Calcein-AM (green) and propidium iodide (red), respectively. e Relative cell viabilities of LLC1 cells incubated in DMEM, PMAA-Fe and Cyp-PMAA-Fe@MSCs with/without 808 nm laser. f Confocal microscopic images of marker of DNA breaks (γ-H2AX) in LLC1 cells treated with PMAA-Fe@MSCs with/without X- ray. g Clonogenic survival assay of LLC1 cells incubated with Cyp-PMAA-Fe@MSCs under different radiation doses