Fig. 9

Apoptosis mechanism related protein detected by western blotting, immunofluorescence and immunohistological staining. a TUNEL assay of apoptosis in tumours and the immunofluorescent detection of tubulin, cleaved-caspase3 and HSP70; b, c apoptosis cell counts of TUNEL and IOD value of tubulin, cleaved-caspase 3 and HSP70; d, e Effect of NIR treatment on the inhibition of tumour-associated macrophages M2 polarization by western blotting; and f–h The immunohistological staining of CD206 in tumours. (^**p < 0.01, ^***p < 0.001)