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Fig. 2 | Journal of Nanobiotechnology

Fig. 2

From: Correction to: M2 Macrophagy-derived exosomal miRNA-5106 induces bone mesenchymal stem cells towards osteoblastic fate by targeting salt-inducible kinase 2 and 3

Fig. 2

M2D-Exos induce osteoblast activity and matrix mineralization. a Osteogenic genes were upregulated in M2D-Exos-treated BMSCs measured by western blotting analysis; b The relative intensity of western blotting analysis; c Overexpression of the four osteognic genes can be detected in M2D-Exos groups measured by qRT-PCR analysis; d Alizarin red-mediated calcium staining in BMSCs following treated by PBS (control group), M1D-Exos, and M2D-Exos for 21 days. Scale bar = 10 mm; e ALP staining in BMSCs following treated by PBS (control group), M1D-Exos, and M2D-Exos for 14 days. Scale bar = 10 mm; f, g The statistical data of Alizarin red-mediated calcium staining and ALP staining. Data are mean ± SD of triplicate experiments. *p < 0.001, **p < 0.01, ***p < 0.001

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