Fig. 2

Administration of GO by oral gavage aggravated DSS-induced colitis in mice. a Schematic illustrations of experiments on the intestinal toxicity of mice after repeated oral exposure to GO. Mice were sacrificed on day 9 to collect colon samples. b Weight changes and c, d colon length were measured. Body weight was presented as the percentage of the initial weight (on day 0). e Representative H&E images of colon tissues. Scale bar: 200 μm. f The total histological score was calculated as the sum of the score for epithelial damage and the infiltration score. Data are presented as the means ± SEMs from three independent experiments in all analyses. *P < 0.05, **P < 0.01