Fig. 2

Generation of LbL-HFSCs based on the LbL cell nanocoating technique. a Schematic diagram of coating human HFSCs to construct LbL-HFSCs. b Images of confocal laser scanning microscopy (CLSM) show the HFSCs coated with gelatin-FITC (green)/alginate-rhodamine B (red). The cells are in suspension. DAPI (blue). Scale: 10 µm. c The cell diameter after different nanocoating layer was calculated using Image-Pro Plus 6.0 based on the images of CLSM (mean ± SD; n = 4; NS, not significant; p > 0.05, one-way ANOVA). d CLSM images displaying the existence of the biomaterials coated on the surface of the HFSCs by LBL. The cells are in suspension. DAPI (blue); Gelatin-FITC (green); scale: 10 µm. e Zeta-potential changes per cell surface coating layer (mean ± SD; n = 4): (0) uncoated HFSCs, (1) gelatin-coated HFSCs, (2) gelatin/alginate- coated HFSCs, (3) (gelatin) 2/alginate- coated HFSCs