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Fig. 3 | Journal of Nanobiotechnology

Fig. 3

From: Nanoscale microenvironment engineering for expanding human hair follicle stem cell and revealing their plasticity

Fig. 3

The effect of LBL coating on HFSCs’ viability, proliferation, or morphology. a Live/dead staining shows the dead cells in the uncoated HFSCs and LbL-coated HFSCs on days 3 and 7 of culture. Dead (red); Live (green); Scale bars: 100 µm. b There was no significant difference in the proportion of dead cells between uncoated HFSCs and LbL-HFSCs (mean ± SD; n = 4; NS, not significant; p > 0.05, Student’s t-test). c Colony formation assays detected the effect of LbL coating on the proliferation of HFSCs. d Number of clones between the uncoated HFSCs and LbL-coated HFSCs. No significant difference was observed (mean ± SD; n = 4; NS, not significant; p > 0.05, Student’s t-test). e The morphology of uncoated HFSCs and LbL-HFSCs on day 1, 3, and 7 was evaluated by SEM. Scale bars: 10 µm. f On days 1 and 3 after coating, the stretched area of the HFSCs was calculated using Image-Pro Plus 6.0 based on the SEM results. The stretched area of the LbL-coated HFSCs was lower than that of the uncoated HFSCs; however, on day 7, there was no significant difference (mean ± SD; n = 4; NS, not significant; p > 0.05, **p < 0.01, Student’s t-test)

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