Fig. 5

FGF-7 and VEGF-A stabilize the CD200⁺α6⁺ HFSC population. a, b Flow cytometry conducted on the seventh day after culture at P2. The ratio of CD200⁺a6⁺ HFSCs in the LbL-HFSCs + FV group was higher than the other groups (mean ± SD; n = 3; **p < 0.01, one-way ANOVA). c, d Immunofluorescence staining performed on the seventh day after culture at P2. Red-stained CD200⁺ HFSCs in the LbL-HFSCs + FV group was higher than the other groups (mean ± SD; n = 4; **p < 0.01, one-way ANOVA). DAPI (blue); CD200 (red); scale bars: 20 µm. e Absolute numbers of CD200⁺a6⁺ HFSCs at P2 cultured under different conditions, indicated as fold-change relative to the number of cells inoculated at P0 (mean ± SD; n = 4; **p < 0.01, one-way ANOVA). f The results of the long-term cell culture performed under different conditions are from flow cytometry analysis of cells in each passage. Only the LbL-HFSCs + FV culture maintained a stable CD200⁺a6⁺ HFSC population for an extensive period after P2 (mean ± SD; n = 3)