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Fig. 6 | Journal of Nanobiotechnology

Fig. 6

From: Nanoscale microenvironment engineering for expanding human hair follicle stem cell and revealing their plasticity

Fig. 6

LbL loading BMP2 mediates CD200+α6+ HFSC population. a Schematic diagram of LbL(BMP2)-HFSC construction. b Immunofluorescence staining against BMP2, confirming the presence of BMP2 on the cell surface of LbL(BMP2)-HFSCs only. BMP2 (green), scale: 20 μm. c The release profile of BMP2 conducted using enzyme-linked immunosorbent assay (ELISA) at different time points after coating (mean ± SD; n = 4). d, e Flow cytometry performed on the seventh day after P2 culture. The proportion of CD200+a6+ HFSCs in the LbL(BMP2)-HFSCs + FV group was higher than the other groups (mean ± SD; n = 3; *p < 0.05, **p < 0.01, one-way ANOVA). f, g Immunofluorescence staining performed on the seventh day after P2 culture. Number of red-stained CD200+ HFSCs in the LbL(BMP2)-HFSCs + FV group was higher than the other groups (mean ± SD; n = 4; *p < 0.05, **p < 0.01, one-way ANOVA). DAPI (blue); CD200 (red); Scale bars: 20 µm

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