Fig. 8
Vital staining of normal human dermal fibroblasts (NHDF) cultured in 3D Collagen/Chitosan based scaffolds enriched by SeNPs of different concentrations (0.5, 1.0, 5.0 μg/ml) or enriched by SeNPs of different concentrations (0.5, 1.0, 5.0 μg/ml) and by FGF2-STAB® of concentration 1 μg/ml for 1 and 3 days. Alive NHDF were stained by calcein-AM (1 μg/ml, 30 min, 37 °C, 5% CO2) (green) and dead NHDF in scaffolds were visualized by PI (1 μg/ml, 30 min, 37 °C, 5% CO2) (red). Pictures were taken by Olympus IX83 in confocal mode with 4 × objective. Scale bar indicates 100 μm