Table 4 Workflow scheme
No | Material | c (SeNPs) (µg/ml) | c (FGF2-STAB®) (µg/ml) | Analysis | Summary |
|---|---|---|---|---|---|
1 | Collagen/Chitosan scaffold | 0; 2; 10; 20 | 0; 2 | AB assay CAM assay | Antagonistic effect of SeNPs and FGF2-STAB® at high concentrations of SeNPs (10 and 20 µg/ml) |
2 | Solution | 0; 1; 5; 10; 50; 100 | / | AB assay IC80 calculation | IC80 is 5.6 µg/ml of SeNPs for HDF |
3 | Solution | / | 0; 0.01; 0.05; 0.1; 0.5; 1 | AB assay | Positive effect on fibroblast metabolic activity at a concentration as low as 0.01 µg/ml |
4 | Solution | 1; 10 | 0; 0.01; 0.1; 1 | AB assay | Synergistic effect of SeNPs and FGF2-STAB® was confirmed in solution; the best combination of agents is 0.01–1 µg/ml of SeNPs and 0.01–1 µg/ml of FGF2-STAB® |
0; 0.01; 0.1; 1; 10 | 0.1; 1 | ||||
5 | Collagen/Chitosan scaffold | 0; 0.5; 1; 5 | 0; 1 | AB assay, Life/dead assay Broth method Morphology and pore size analysis | Optimal concentration for Collagen/Chitosan scaffold enrichment is 0.5 µg/ml of SeNPs and 1 µg/ml of FGF2-STAB® |