Fig. 2

Characterization of BMSCs-derived exosomes. A The morphology of the exosomes and PGZ-Exos was visualized with TEM. Scale bar: 50 nm. B The exosome marker proteins CD9 and CD63 in the exosomes and PGZ-Exos were detected with western blotting. C The size distribution of exosomes and PGZ-Exos was examined with NTA. D PKH26-labeled exosomes and PGZ-Exos uptake by HUVECs was investigated with laser scanning confocal microscopy. The cytoskeleton, exosomes and cell nucleus are stained green, red, and blue respectively. Scale bar: 10 µm