Fig. 3

PGZ-exos protected against HG-induced inhibition of HUVECs viability and cell proliferation. A CCK8 assay was performed to assess HUVECs viability after treatment with HG and exosomes. B, C EdU incorporation assay was used to evaluate HUVECs proliferation. Scale bar: 50 µm. D, E Flow cytometry was used to assess the cell cycle distribution of the HUVECs. F, G Western blotting was used to investigate the protein expression of Cyclin D1, Cyclin D3 and VEGF. Data are presented as means ± SD from three independent experiments. *P < 0.05 versus the control (con) group