Fig. 9

Immunohistochemical analysis of diabetic wounds post-surgery. A Representative immunohistochemical analysis images of collagen I, collagen III. Scale bar: 50 μm. B, C Quantification of the positive areas of the collagen I and collagen III among the different groups using the ImageJ software. D Representative immunohistochemical analysis images of VEGF (Scale bar: 50 μm) and CD31 (Scale bar: 100 μm). E Quantification of the positive areas of VEGF among the different groups using the ImageJ software. F Quantification of the number of CD31 positive among the different groups using the ImageJ software. Data are presented as means ± SD from three independent experiments. *P < 0.05 versus the control (con) group