Fig. 2

Downregulation of S1PR1 improves the chemosensitivity of BRCA and inhibits vascular formation by decreasing P-STAT3/VEGFA. a–c qPCR analysis of S1PR1 expression in MCF-7 and MDA-MB-231 and HUVECs after treated with S1PR1-siRNA for 48 h. Mean ± SEM, ***P < 0.001. d–f Western blotting assays show the S1PR1, P-STAT3 and VEGFA protein expression in MCF-7 and MDA-MB-231 cells and HUVECs after treatment with S1PR1-siRNA for 48 h. g–j MTT analysis of the viabilities of MCF-7 and MDA-MB-231 cells following treatment with the cisplatin or doxorubicin for 2 days. k Vascular formation assay analysis of the tube formation of HUVECs pretreated with S1PR1-siRNA for 20 h. l The statistical results show the tube formation number of HUVECs. Mean ± SEM, **P < 0.01