Fig. 6
Evaluation of the motor development biomarkers A distance traveled and B speed for zebrafish (Danio rerio) larvae exposed to the different treatments: control, ascorbic acid (AA), CS nanoparticles (NPs_CS), CS nanoparticles containing ascorbic acid (NPs_CS_AA), PCL nanoparticles (NPs_PCL), and PCL nanoparticles containing ascorbic acid (NPs_PCL_AA). The exposures were performed for 96 h at different concentrations (LC10, LC20, LC30, LC40, and LC50). For the statistical tests, ANOVA (two-way, significance of p < 0.05) was used to evaluate differences among the groups, with α, β, δ, σ, and λ indicating statistically significant differences from the control, LC10, LC20, LC30, and LC40 groups, respectively (n = 9)