Ecotoxicity evaluation of polymeric nanoparticles loaded with ascorbic acid for fish nutrition in aquaculture

Table 2 Average lethal concentrations (mg/L) for zebrafish embryos and larvae exposed to different treatments: ascorbic acid (AA), chitosan nanoparticles (NPs_CS), chitosan nanoparticles containing ascorbic acid (NPs_CS_AA), PCL nanoparticles (NPs_PCL), and PCL nanoparticles containing AA (NPs_PCL_AA)

LC – 96 h (mg/L)	AA	NPs_CS	NPs_CS_AA	NPs_PCL	NPs_PCL_AA
LC₅₀	330.7	28.9	57.4	168.9	179.6
LC₄₀	275.8	24.8	48.1	137.5	146.1
LC₃₀	217.1	20.3	38.1	103.8	110.2
LC₂₀	148.3	15.2	26.4	64.5	68.2
LC₁₀	53.0	8.0	10.2	9.9	10.0

The concentrations tested for each exposure were LC₁₀, LC₂₀, LC₃₀, LC₄₀, and LC₅₀. The LC values after 96 h of exposure were determined by probit analysis, employing Statgraphics Centurion XVII v. 1.17.04 software (Stat Point Technologies, 2014). The plates were analyzed using a stereomicroscope (Model SMZ 2 LED, Optika), at 2 × magnification

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