Fig. 2

Measurement process of κ for an MT of L = 10 µm and κ_set = 0.3 × 10⁻²³ N·m² with different localization precision. MT shapes (blue lines) and fitted curves (red lines and dots) with localization precision of A 1 nm and B 100 nm. Relationship between q_n⁴ and L⁴/var(a_n) with localization precision of C 1 nm and D 100 nm. Blue dots: measurement results. Red lines: fitted straight lines