Fig. 5

(DOC + E6E7) @DOTAP reduces cell viability and induces cell apoptosis. A HeLa (HPV18 positive) cells were treated with (DOC + E6E7) @DOTAP or control for 48 h before protein expression of various growth control proteins (p53 for E6 targeting, RB for E7 targeting) was determined by western blot analysis. B The relative density of bands was quantified using ImageJ software. C–E CCK-8 assay, cell apoptosis assay and Calcein-AM/PI staining. Cells were treated with the nanosystem for 48 h before cell apoptosis assay and Calcein-AM/PI staining. Scale bars, 200 μm. F E6 and E7 mRNA expression in the HeLa cells. *p < 0.05, **p < 0.01 vs. control