Fig. 5

Comparison of structure and luciferase silencing of WRAP-based PBNs and their analogues. A Ribbon presentation of the predicted 3D structure of the WRAP peptides and WRAP analogues by PEPstrMOD in a hydrophilic environment. B siRNA-loaded WRAP analogues were evaluated for their luciferase silencing activity in comparison to the parental WRAP PBNs. Graphical representation highlighted the relative Luc activity (%) and relative cytotoxicity (LDH quantification, %) after transfection with WRAP:siLuc complexes on U87 cells. Conditions: WRAP:siRNA (R = 20) at the indicated siRNA concentrations. Abbreviations: siLuc = firefly luciferase siRNA, N.T. = non-treated cells, Ctrl = Controls. Data represent mean ± SD, with n = 2 independent experiments in triplicate