Fig. 8
Treatment effect of NPs. a The treatment protocol. b Plots of body weight at week 9 after treatment (n = 8). c Plot of uterine wet weight in different groups at week 9 after treatment (n = 9). *P < 0.05 versus free E2, #P < 0.05 versus PEG-PLGA-AL@E2 NPs. d Representative three-dimensional trabecular architecture of the distal femur from the respective groups obtained by micro-CT examination at week 9 after treatment. Treatment groups TG1: OVX, TG2: free E2, TG3: PLGA@E2 NPs, TG4: AL-PLGA@E2 NPs, TG5: PEG-PLGA@E2 NPs, TG6: PEG-PLGA-AL@E2 NPs, TG7: PEG-PLGA-AL@Fe3O4 NPs, TG8: PEG-PLGA-AL@Fe3O4/E2 NPs, TG9: PEG-PLGA-AL@Fe3O4/E2 NPs + MF