Fig. 4

Uptake of C6-labeled nanocarriers by A A549 and B RAW264.7 cells. HA&RBCm-LCNPs-1 and -2 refer to LCNPs with HA-DOPE concentrations of 0.5 and 1.0 mg/mL, respectively. The “Control” group represents untreated cells; the “with free HA-DOPE” group was administered HA&RBCm-LCNPs without the removal of free HA-DOPE during the final process. Analysis was performed through flow cytometry (1; top, FACS analysis of the C6 signal; bottom, statistical analysis of differences in the fluorescence intensity among the groups) and confocal laser scanning microscopy (2; nucleus was labeled with DAPI) (n = 3). ****p < 0.0001