Fig. 4
In vitro permeability across mucus layer and Caco-2 cell monolayer. A Schematic illustration of the transwell experiment. Mucin (10 mg/mL) was added to the upper chambers (thickness of 500 μm), and the lower cambers were filled with HBSS. Transportation of B BER and C CUR across mucus layer in different preparations. Each bar represents the mean  ±  SD (n  =  3). *p  <  0.05, **p  <  0.05 and ***p  <  0.001 vs BER  +  CUR; #p  <  0.05 and ###p  <  0.001 vs DEAE-DEX@LSDBC; §p  <  0.05 LBC vs LSDBC. D Papp values of various preparation groups in Caco-2 cell monolayers. Each bar represents the mean  ±  SD (n  =  3). ***p  <  0.001 vs BER  +  CUR; ###p  <  0.001 vs DEAE-DEX@LSDBC. E Relative TEER changes of the Caco-2 cell monolayers in different groups. After 2 h incubation, the preparations were removed from the Caco-2 cell monolayers. Each bar represents the mean  ±  SD (n  =  3). F Papp values of the DEAE-DEX@LSDBC in Caco-2 cell monolayers under different inhibitory conditions. Each bar represents the mean  ±  SD (n  =  3). *p  <  0.05, **p  <  0.01 and ***p  <  0.001 vs Control