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Fig. 4 | Journal of Nanobiotechnology

Fig. 4

From: Elaboration on the architecture of pH-sensitive surface charge-adaptive micelles with enhanced penetration and bactericidal activity in biofilms

Fig. 4Fig. 4

Zeta potential changes of S. aureus and E. coli after incubation with ML-E-A and ML-A-E under pH 7.4 or 5.5 for different periods of time, respectively (A). CLSM images of S. aureus and E. coli incubated with Nile Red loaded ML-E-A and ML-A-E for 1 h, 2 h, 4 h and 8 h, respectively (B). The mean gray value of CLSM images measured by Fiji ImageJ (C). Quantitative cellular uptake of ML-E-A/Nile red and ML-A-E/Nile red in S. aureus and E. coli evaluated by flow cytometry at pH 7.4 and 5.5 for 1 h, 2 h, 4 h and 8 h, respectively (D). BLI assay for the interaction between b-LPS and ML-E-A (pH 7.4), ML-A-E (pH 7.4), ML-E-A (pH 5.5) and ML-A-E (pH 5.5) (E). CLSM images of biofilms penetration of ML-E-A/Nile red and ML-A-E/Nile red at pH 5.5 and 7.4 after 1 h, 2 h and 4 h of incubation, respectively. Scale bars = 100 μm (F)

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