Table 1 Chemical and biological reagents used in this work
Reagent | Brand & purity |
|---|---|
3T3 and caov-4 cell lines | ATCC |
(3-Chloropropyl)trimethoxysilane (CPTMS) | Sigma-Aldrich, ≥ 97.0% |
Crystal violet (tris(4-(dimethylamino)phenyl)methylium chloride) | Merck, ≥ 90.0% |
Docetaxel (DTX) | Sigma-Aldrich, purum, ≥ 97.0% (HPLC) |
Dulbecco’s modified Eagle’s medium (DMEM) | Sigma-Aldrich |
Ethylene glycol | Sigma-Aldrich, spectrophotometric grade, ≥ 99% |
Halloysite nanotubes (HNTs) clay | Sigma-Aldrich |
Lugol’s solution (iodine 5%, and potassium iodide 10% w/v) | Merck |
MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) | Sigma-Aldrich, 98.0% |
Paper filter | Whatman (602 h, particle retention < 2 µm) |
Deionized water | Merck |
Ethanol | Merck, 96%, (synthesis grade) |
Toluene | Merck, (chromatographic grade) |
Dimethyl sulfoxide (DMSO) | Merck, (analysis grade) |
Sortilin 2D8-E3 mAb (SORT) | Avicenna Research Institute, Iran |
Sodium borohydride | Sigma-Aldrich, 98.0% |
Tetrachloroauric(III) acid trihydrate | Sigma-Aldrich, Supelco, 99% |
Trisodium citrate dihydrate | Sigma-Aldrich, ≥ 99.0% |