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Fig. 1 | Journal of Nanobiotechnology

Fig. 1

From: Tetraspanins are unevenly distributed across single extracellular vesicles and bias sensitivity to multiplexed cancer biomarkers

Fig. 1

Characterization of SK-OV-3 EVs isolated from bioreactor conditioned media. a TEM micrograph of isolated EVs. b Western blot of EV associated tetraspanins CD9, CD63, and CD81. Size distribution was assessed by either (c) NTA (3 × 30 s acquisitions), d TEM (3 images with ≥ 50 particles), or (e) SP-IRIS (anti-CD81 capture) (3 capture spots). The distributions, modes, and apparent concentrations varied between the three techniques. Error bars represent ± one s.d

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