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Fig. 2 | Journal of Nanobiotechnology

Fig. 2

From: Tetraspanins are unevenly distributed across single extracellular vesicles and bias sensitivity to multiplexed cancer biomarkers

Fig. 2

ExoView platform for particle capture and analysis. Immuno-captured EVs are characterized by (a) immunofluorescence and (b) SP-IRIS. Images captured in either (c) fluorescence mode (blue: CF488-anti-CD9, red: CF647-anti-CD63, green: CF555-anti-CD81) or (d) SP-IRIS mode can indicate detected particles (blue circle diameter scaled to estimated EV diameter). e Serially diluted EVs were incubated on chips, with captured EVs detected by SP-IRIS (circles) or immunofluorescence (diamonds). Comparing counts between the two cases revealed a discrepancy in limit of detection, with fluorescence yielding higher sensitivity. f To find the optimal incubation concentration where signal was maximized for single particles, fluorescence intensity per particle was calculated. Arrows indicate the highest concentration with reliable single-particle detection, above which “swarming” occurs. Error bars represent ± one s.d. across 3 capture spots. *p < 0.05 by Student’s t-test

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