Fig. 4

Comparison of tetraspanin profile of EVs isolated by UC from varying biofluid source. Representative EVs derived from (a) SK-OV-3, b serum, and c PMSCs exhibit distinct tetraspanin profiles. N = 3 technical replicates representing 3 capture spots each. Three samples of EVs from each source were analyzed and the average fraction of total fluorescent particles was determined for detection antibody on (d) any capture spot or (e) single capture spot. This highlights the complex nature of tetraspanin multiplexing. N  =  3 experimental replicates representing 3 capture spots each. Error bars represent ± one s.d. * p  <  0.05, ** p  <  0.01, *** p  <  0.001 by ANOVA