Skip to main content
Fig. 7 | Journal of Nanobiotechnology

Fig. 7

From: Tetraspanins are unevenly distributed across single extracellular vesicles and bias sensitivity to multiplexed cancer biomarkers

Fig. 7

a Biotinylated SK-OV-3 EVs were captured by anti-CD9, anti-CD63, anti-CD81, anti-biotin, or anti-MIgG, and detected by anti-CD24 (green), anti-EpCAM (blue), and anti-Her2 (red). CD24 is only multiplexed with CD9, while EpCAM and Her2 are associated with all three tetraspanins. Anti-biotin captured significantly more CD24 + and Her2 + EVs than any single tetraspanin. N = 3 capture spots. Error bars represent ± one s.d. * p < 0.05, ** p < 0.01, *** p < 0.001 by ANOVA. b Our proposed model, illustrating that specific capture by any given single tetraspanin results in limited co-expression of particular tumor markers as compared to non-specific EV capture via anti-biotin

Back to article page