Fig. 4
From: Imaging asparaginyl endopeptidase (AEP) in the live brain as a biomarker for Alzheimer’s disease
Fluorescence intensity of AuNPs-Cy5.5-A&C in cortical neurons. A Images of cortical neurons after incubation with AuNPs-Cy5.5-A&C or other probes for 24 h. Treatment with Aβ25–35 (5 μM) were for 24 h. Atorvastatin (Ato) at 20 μM was used to inhibit AEP. B The enzymatic activity of AEP in neurons. *P < 0.05, Aβ treatment versus control group. n = 3 independent experiments, Student’s t-test. C Fluorescent intensity of Cy5.5-tagged probes after a 24-h incubation with neurons. *P < 0.05, AuNPs-Cy5.5-A&C in Aβ-treated cells compared with control cells. Student’s t-test, n = 3 independent experiments. **P < 0.01, AuNPs-Cy5.5-A&C compared with other probes in Aβ-treated cells, one-way ANOVA. n = 3 independent experiments, 40–50 cells were imaged per test