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Scheme 1 | Journal of Nanobiotechnology

Scheme 1

From: Imaging asparaginyl endopeptidase (AEP) in the live brain as a biomarker for Alzheimer’s disease

Scheme 1

This scheme was adapted with permission from (ACS Nano 2016, 10, 11, 10086–10098) [26]. Copyright © 2016 American Chemical Society)

The mechanism of aggregation-based fluorescent enhancement. The AuNPs-Cy5.5-AK contained Ala–Ala–Asn–Cys–Lys, which was cut by AEP to expose 1, 2-thiolamino group of cysteine. The click cycloaddition occurred between the 1, 2-thiolamino group of AuNPs-Cy5.5-AK and cyano group of AuNPs-Cy5.5-CABT, leading to aggregation of AuNPs and strong fluorescent emission. Inset showing structures of SH-PEG-CABT and SH-PEG-AK and the splice site.

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