Fig. 3

BMEVs induce the apoptosis of OSCC. a TUNEL staining assay of CAL 27 and WSU-HN6 cells after BMEVs treatment, scale bar = 100 μm; b Western blot analysis of cleaved caspase 3 protein expression; c CAL 27 and WSU-HN 6 cells were stained with Annexin-v and 7-AAD to analyze apoptosis rates by flow cytometry; d representative column graph analysis of the apoptosis rate; e fluorescence images of live/dead stained 3D cultured cells, scale bar = 100 μm