Fig. 2
Cellular imaging, localization, and uptake. The OPE/TPE confocal images (a) of Ir-NPs distribution in Skov3 cells, scale bar: 10 μm. CLSM observation (b) and flow cytometer (c) of Ir-NPs uptake by Skov3 cells for 2, 4, and 6 h, respectively, scale bar: 50 μm. The uptake inhibition percent analysis (d) of Ir-NPs uptake by Skov3 cells upon different treatments of endocytosis inhibitors (CPZ, EIPA, Mβ-CD, and filipin III) at 37 °C and 4 °C, ****P < 0.0001. Ir-1: λex = 405 nm (OPE)/810 nm (TPE), λem = 590 ± 20 nm, DAPI: λex = 405 nm, λem = 440 ± 20 nm Mito-tracker Green (MTG): λex = 488 nm, λem = 510 ± 20 nm