Fig. 2
TAT-Cx43266–283 reduces the invasion of GL261 glioma cells in vivo. Matrigel invasion assay. GL261 cells were treated with 100 µM TAT or TAT-Cx43266–283 for 15 h (A). Mosaic immunofluorescence of DAPI (blue), mCherry GL261 glioma cells (red), the thresholded images (bottom, black), the tumor rims (top), and their magnifications (B). GL261-GSCs together with 100 µM TAT-Cx43266–283 or saline were intracranially injected in C57BL/6 mice. After 7 days, a twice per week i.p. injection of saline or 4 nmol/g TAT-Cx43266–283 was administered until neurological symptoms appeared (C). Effect of TAT-Cx43266–283 on the survival of mice bearing orthotopic tumor syngrafts. Percentage of animals alive along the experiment depicted in Kaplan–Meier plot (n = 11 animals per condition from 3 independent experiments). Log-rank test **P < 0.01 (D) Representative images of the brains and tumor-bearing brain sections from control and treated animals at the end of the experiment. Bar: 1 mm (E). Reprinted with permission from ref. [89] Copyright (2019) Oxford University Press JaraÃz-RodrÃguez et al.