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Fig. 3 | Journal of Nanobiotechnology

Fig. 3

From: Development of theranostic dual-layered Au-liposome for effective tumor targeting and photothermal therapy

Fig. 3

In vitro PTT results of AL and LAL. Cell viability of 4T1 treated AL and LAL a without laser and b with the 2 W cm−2 laser irradiation for 5 min (n = 4, mean ± s.d.). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. Data were analyzed by one-way ANOVA with Tukey’s post-test. c Confocal images of AL and LAL internalized in 4T1 cells. All scale bars in the images are 50 μm. TD: transmitted light channel, blue: nuclei (Hoechst 33342), green: cytoskeleton (ViaFlour® 488), red: LAL or AL (R6G). d The quantified fluorescence intensity of each cell where AL or LAL internalized (n = 14, mean ± s.d.). Data were analyzed by Student’s t-test. *P < 0.05. e The fluorescence images representing 4T1 cell nuclei to observe DNA-DSBs by the γ-H2AX foci after the laser irradiation (808-nm laser, 2.5 W cm−2, 5 min). Blue: nuclei (Hoechst 33342), green: γ-H2AX focus (Alexa Flour 488). All scale bars in the images are 10 μm. f DNA-DBSs quantitative analysis in 4T1 cells with and without laser irradiation (n = 5, mean ± s.d.). *P < 0.05, ***P < 0.001. Data were analyzed by one-way ANOVA with Tukey’s post-test

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