Fig. 4

In vivo fluorescence images and assessment of passive tumor targeting efficacy of DiR labeled LAL and AL in 4T1 tumor bearing mouse model (n = 4). a In vivo fluorescence images of 4T1 breast cancer bearing mouse model at 0, 2, 12, and 24 h. DiR labeled LAL (left column) and AL (right column) injected mice. White arrows indicate tumors. b Representative ex vivo fluorescence images of tumors and main organs (He: heart, Li: liver, Sp: spleen, Ki: kidney, and Lu: lung) resected from each mouse after in vivo imaging. Comparison of tumor to non-targeted organ, c liver, d spleen, and e lung ratio. Tumor targeting efficiency comparison with non-targeted organ, c liver, d spleen, and e lung. Data were analyzed statistically with a Student’s t-test. *P < 0.05, ***P < 0.001, ****P < 0.0001