Fig. 5From: Formulating RALA/Au nanocomplexes to enhance nanoparticle internalisation efficiency, sensitising prostate tumour models to radiation treatmentQuantification of DNA double strand breaks following radiation and nanoparticle treatment. Cells seeded in chamber slides were treated with citrate-AuNP (1 µg) or RALA-AuNP (25 µg RALA: 1 µg AuNP) for 6 h. Excess AuNPs were removed, cells washed and then irradiated with 1 Gy. Cells were fixed and stained with anti-53BP1 and DAPI 30 min and 24 h post irradiation. Slides were imaged using a Lecia SP8 confocal microscope using × 40 objective. Z-stacks were taken at 0.5 µm steps with a minimum of 12 sections acquired. A Representative images of three independent replicates for PC-3 cells. Quantified 53BP1 foci indicating double strand break lesions in: B DU145, C PC-3 and D PNT2-C2 cellsBack to article page