Fig. 1

Lentiviral expression vectors affect the function of some lncRNAs. A Screen for lncRNAs whose function may be affected when overexpression with lentivirus vectors. B The expression levels of lncRNAs expressed by pCDH in HepG2 cells were tested using qRT-PCR. C The effects of lncRNAs expressed by pCDH on the cell proliferation were tested using colony formation assay in HepG2 cells. D A simplified schematic diagram shows the mechanism of lentivirus vector pCDH in transcriptional termination of exogenous expressed lncRNAs. E A simplified principle of transcriptional termination of exogenous expressed lncRNAs of ELECTS. F, G 3ʹ RACE showed the lengths of HOTAIRM1 (F) and HCCL5 (G) transcribed using ELECTS and pCDH respectively. H Northern Blot revealed the lengths of the exogenous HOTAIRM1 products expressed using ELECTS and pCDH. I RNA-seq results showing the signal of HOTAIRM1 transcripts produced by ELECTS (top panel) and pCDH (bottom panel), the reads were mapped to the corresponding vector sequences