Table 1 Comparison of ELECTS and other available vectors for stable expression of lncRNAs
vectors | ELECTS | Lentivirus | pcDNA |
|---|---|---|---|
Integration rate | ++++ | +++ | ± |
Expression cassette after integration | Intact | Intact | Mostly partial |
Transcription termination site | BGH | 3ʹLTR | BGH |
Length of lncRNA product | As endogenous | Flanked by extra sequences | As endogenous |
Secondary structure of lncRNA | Native | May be altered | Native |
Function of lncRNA | Normal | May be altered or abolished | Normal |
Time | ~ 1 week | 10–14 days | Up to 6 months |
Biosafety | safe | Potential risk | safe |