Fig. 5
From: Microfluidics applications for high-throughput single cell sequencing
Comparison of droplet generation, emulsion, and library preparation and sequencing on the microfluidics-based scRNA-seq methods. A inDrop lyses single cells and then barcodes their mRNA with barcoded hydrogel microspheres in droplets; B Drop-seq applies barcoded beads capturing single cell mRNA and then released from the drops and performed reverse transcription; C 10× Genomics uses Gel bead in EMulsion (GEM) for encapsulating thousands of single cells, and then immediately lysis cells for reverse transcription