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Fig. 7 | Journal of Nanobiotechnology

Fig. 7

From: Microfluidics applications for high-throughput single cell sequencing

Fig. 7

Application of microfluidic in immune system diseases. A These selectively labels peptide antigen-specific CD8 + T-cells are isolated in the microfluidic device integrated a DLD array for MATE-seq. Barcoded cells are encapsulated in water-in-oil droplets with lysis RT-PCR mixture, and collected for analysis. Adapted with permission from Alphonsus et al. [151]; B A flow-focusing droplet generator was used to generate aqueous in oil droplets containing co-encapsulated TCR T cells and target cells, which contributes to real-time monitoring of single TCR T cell activation after recognition of target tumor cells. Adapted with permission from Segaliny et al. [152]; C The droplet production, droplet sorting and co-compartmentalization of single cells with single beads in droplets are integrated in the CelliGO system, which is used for high-throughput single-cell activity-based screening and sequencing of antibodies. Adapted with permission from Annabelle et al. [154]

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